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Measuring impact at scale Lincoln leaching Lincoln establishment and persistence Lincoln production Lincoln economics Massey establishment and persistence Massey leaching Massey production Plantain potency and practice programme Publications Additional resources

As part of the DairyNZ-led Plantain Potency and Practice programme, trials at Lincoln and Massey universities tested how plantain affects nitrogen leaching, pasture persistence and farm productivity under different soil and climate conditions.

At Lincoln, pastures containing an average of 17% Ecotain® plantain reduced annual nitrate leaching by an average of 33% and annual total nitrogen leaching by an average of 17% over four years, with no significant effect on milksolids production or profit.

At Massey, pastures with plantain reduced annual nitrogen leaching by 20-60% in the first two years. Results in later years were influenced by seasonal conditions, pasture composition and lower overall leaching risk.

Across both sites, the research shows plantain can significantly reduce nitrogen losses while maintaining milk production.

The Lincoln and Massey sites also hosted a range of experimental work covering nitrous oxide emissions, methane emissions, plantain pasture management, animal health and effects of plantain on animal urine, milk and blood. Publications from these studies are provided at the end.

Measuring plantain impact on leaching and productivity at scale

Plantain impact was assessed through trials at Lincoln and Massey universities, representing free-draining irrigated soils and heavy soils under high rainfall conditions, respectively.

At Lincoln University Research Dairy Farm (2021-2025), ryegrass/white clover pastures (PL0) were compared with ryegrass/white clover/plantain mixed pastures sown with 3 kg/ha (PL3) and 6 kg/ha (PL6) plantain (seed mixes below) in small pasture-based farm systems (‘farmlets’ of 3.6 ha and 12 cows). Each treatment was replicated three times, giving a total of nine farmlets.

Farmlets were managed individually, and farm productivity and profitability were measured throughout the study. Leaching was measured using ceramic suction cups under the PL0 and PL3 treatments. The volume of water draining through the soil surface was calculated based on rainfall and irrigation using a water balance model.

At Massey University No. 4 Dairy (2020-2024), large, grazed plots with ryegrass/white clover pastures were compared with mixed ryegrass/white clover/plantain swards with low, medium and high plantain content in a high input system with supplement making up around 30% of the diet. Leaching was measured by capturing all drainage water through mole drains isolating each plot. Cows simultaneously grazed plots of every treatment (following a seven-day transition period) before completing their round on the rest of the farm. Production parameters were measured on the days cows were grazing the treatment plots.

Watch the video below for more about the Massey trial.

Lincoln Massey Plantain Measuring Nitrogen Image
Lincoln Massey Plantain Measuring Nitrogen Image

Leaching was measured using ceramic suction cups.


736 ceramic suction cups were installed at 600mm depth under the PL0 and PL3 paddocks at Lincoln to measure the concentration of nitrogen in drainage water.

Massey Plantain Video Placeholder Image

Video 07:00 min

Lincoln leaching results

Results showed that, over the four-year trial, the PL3 treatment, which contained an average of 17% plantain in sward dry matter, reduced annual nitrate leaching by an average of 33% compared with PL0 (ryegrass/white clover without plantain). Annual total nitrogen leaching was also reduced by an average of 17% (Table 1). There was no significant treatment by year effect, i.e. the differences between treatments persisted across the years.

Table 1. Annual average nitrate and total nitrogen leaching losses at Lincoln plantain farmlet trial.

Average (2022-2025) Nitrate + Nitrate Kg/ha/yr leached Total N (mineral N + organic N) Kg/ha/yr leached
Perennial ryegrass/clover (PL 0) 18 (14-24) 71 (64-79)
Perennial ryegrass/clover/plantain (PL3) 12 (10-16) 59 (53-65)
% reduction 33% 17%
P value (treatment effect) 0.028 0.016
P value (treatment by year effect) 0.599 0.962

Note: Results are presented as back-transformed least square means with 95% confidence interval in brackets. These results are preliminary as we await development of peer-reviewed publications.

Aside from nitrate, total nitrogen includes ammonium and organic nitrogen. Treatment differences in these latter nitrogen forms were small and not significant with an 8% reduction in ammonium N (P = 0.231) and 7% reduction in organic N (P = 0.144). Organic N leaching was 43 kg/ha/yr in the ryegrass/clover and 39 kg/ha/yr in the ryegrass/clover/plantain.

Lincoln plantain establishment and persistence

Pastures were established with Legion perennial ryegrass, Tribute white clover and Ecotain® plantain in March 2021. Over the four years, plantain content was maintained with over-sowing and under-sowing of Ecotain® plantain (Table 2).

Annual average and autumn plantain contents as a proportion of total dry matter are provided in Table 2. Autumn is the recommended timing for assessing plantain as this is the highest risk period for leaching. The data shows that plantain levels peaked in Autumn 2022 before declining and then stabilising at 12-13% in PL3 and 14-16% in PL6.

Table 2. Average annual and autumn plantain % in sward dry matter and associated sowing interventions of Ecotain® throughout the trial.

Year Av. PL6
(% DM)
Aut. PL6
(% DM)
Av. PL3
(% DM)
Aut. PL3
(% DM)
2021/22 34 46 24 34
  Plantain sowing and oversowing
March 2021:
PL6 = establishment with 15kg/ha PRG, 2kg/ha white clover, 6 kg/ha Ecotain®
PL3 = establishment with 18kg/ha PRG, 2kg/ha white clover, 3kg/ha Ecotain®
PL0 = establishment with 20kg/ha PRG, 2kg/ha white clover
February 2022:
Oversown with 6 kg/ha Ecotain® on 1/3 of each plantain treatment area.
2022/23 20 17 16 12
  Plantain sowing and oversowing
February 2023:
Undersown with 6 kg/ha Ecotain® on 1/3 of each plantain treatment area
2023/24 16 19 13 14
  Plantain sowing and oversowing
September 2023:
Oversown all PL6 paddocks with 7kg/ha Ecotain® and all PL3 paddocks with 5 kg/ha Ecotain®
2024/25 16 14 13 12
  Plantain sowing and oversowing
October 2024:
Oversown 1/4 of PL3 area with 5 kg/ha Ecotain®
Average 22% 24% 17% 18%

Note: These results are preliminary as we await development of peer-reviewed publications.

Lincoln milk and herbage production

Milksolids production was similar between treatments (Table 3). Imported silage was higher in the PL6 treatment in 2022/23, associated with lower spring pasture production in PL6 that year. Silage fed in other years was similar between treatments. Nitrogen fertiliser applied was the same across all treatments averaging 149 kg/ha/yr.

Herbage production was measured using multiple methods at the Lincoln site (Table 3). Results from using the rising plate metre with calibration cuts indicated larger treatment differences than using the standard winter equation, which does not account for the lower dry matter percentage of plantain. Using the winter equation, PL6 produced more than PL0 in the first year. With the calibrated equation, differences in the first year were not significant, but PL0 produced more than treatments with plantain in the following years. The back-calculated value from the Farmax model uses milk production and supplement to estimate herbage production. Herbage production was similar between treatments using this method.

At the Lincoln site, a new calibrated equation was developed for the rising plate metre, accounting for lower dry matter percentage of plantain.

Table 3. Milk and herbage production and silage fed over four years on pastures sown with perennial ryegrass/white clover at 0 (PL0), 3 (PL3) and 6 (PL6) kg/ha plantain.

Different letters represent significant treatment differences within a year (P<0.05).

      Herbage production t DM/ha
Year Treatment Milksolids
(kg/ha)
RPM
standard equation
RPM
calibrated
Farmax
back calculation
Imported silage
(t/ha)
2021/22 PL0 1367 12.4a 12.5 14.3 0.38
PL3 1335 12.9ab 13.0 14.3 0.28
PL6 1367 13.6b 12.9 14.7 0.09
2022/23 PL0 1570 14.0 18.7c 16.5 1.56a
PL3 1496 14.2 16.1b 15.8 1.46a
PL6 1503 13.4 14.5a 15.0 2.18b
2023/24 Pl0 1472 13.5 17.3b 16.4 1.78
PL3 1508 13.3 16.3a 16.7 2.00
PL6 1492 13.3 16.2a 15.8 2.15
2024/25 PL0 1554 14.2 16.3b Not available 0.86
Pl3 1543 14.2 15.7ab Not available 1.03
PL6 1533 14.6 15.2a Not available 0.95
P value treatment 0.528 0.789 <0.001 0.553 0.089
P value year <0.001 <0.001 <0.001 <0.001 <0.001
P value treatment x year 0.476 0.003 <0.001 0.147 0.009

Note: These results are preliminary as we await development of peer-reviewed publications.

Estimated marginal means herbage growth rate measured with a rising plate metre with calibration (GR calib) over four production years

Lincoln Massey Plantain Calib Graph 21 22 V2 Lincoln Massey Plantain Calib Graph 22 23 V2 Lincoln Massey Plantain Calib Graph 23 24 V2 Lincoln Massey Plantain Calib Graph 24 25 V2 Lincoln Massey Plantain Calib Graph Average Across Yrs V2

Lincoln economics

An economic analysis using the Farmax model has been completed comparing PL0 to PL3 in Years 1-3 (Table 4). Actual Fonterra milk prices were used – NZD $7.92, $9.26, and $7.80 for Years 1 to 3 respectively (2021/22 – 2023/24). The analysis showed no significant treatment difference in revenue or profit over the three years.

Farm expenses were higher in the PL3 treatment, approximately equivalent to the costs of reseeding in each year (Table 2), which averaged $95/ha. Canterbury farmers partnering in the Plantain programme are maintaining plantain by spreading lower rates of plantain (2 kg/ha/year) with their annual maintenance fertiliser at a cost of around $46/ha.

An analysis of all treatments and years will be completed and published in due course.

Table 4. Profitability of PL0 (perennial ryegrass/clover) compared to PL3 (perennial ryegrass/clover sown with 3kg/ha plantain) for Years 1-3 of the Lincoln Farmlet Trial

Data taken from Hindz 2026

Year Treatment Revenue
(S/ha)
Reseed expenses
($/ha)
Total expenses
($/ha)
Operating profit
($/ha)
2021/22 PL0 11,282   6,836 4,446
PL3 11,023 43 6,873 4,150
2022/23 PL0 14,936   6,822 8,114
PL3 14,306 142 6,942 7,364
2023/24 PL0 12,025   6,733 5,293
PL3 12,304 100 6,871 5,433
P value treatment 0.355   <0.001 0.183
P value year <0.001   0.005 <0.001
P value treatment x year 0.251   0.054 0.274

Massey plantain establishment and persistence

The pasture treatments at Massey were sown in April 2019 and targeted 30% (Low, LPL), 50% (Medium, MPL) and 70% (High, HPL) plantain. Seed mixes and reseeding were applied as per Table 5. The High plantain treatment was resown in April 2021 to achieve the 70% target and was subsequently oversown with Italian ryegrass in March 2023 when clover levels became too high. The actual plantain percentage achieved in each treatment each year was generally lower than target.

Table 5. Sowing rate (kg/ha of seed) at establishment of the study, and reseeding aimed at maintaining the % plantain for each pasture treatment.

Treatments are perennial ryegrass/white clover (RGWC), and targeting 30, 50 and 70% plantain within a RGWC sward (Low, Medium and High PL respectively.

  Pasture treatments
Establishment RGWC LPL MPL HPL
Perennial ryegrass (One50) 20 15 10 5
White clover (Tribute) 3 3 3 3
Plantain (Agritonic) 0 4 7 10
Reseeding1        
March 2020   3 (PL) 6 (PL) 9 (PL)
April 2021       10 (PL) + 3 (WC)
+ 3 (RG)
November 2021   4 (PL) 6 (PL) 8 (PL)
March 2023   4 (PL) 6 (PL) 12 (PL)

1Reseeding (direct drilled) of extra plantain (PL), perennial ryegrass (RG), Italian ryegrass (IR), or white clover (WC)

How resowing affected plantain levels

The graph below shows when resowing occurred and the effect on the average plantain percentage in the sward for each pasture treatment (ryegrass and white clover sward (RGWC; targeting 0% plantain in sward); LPL, MPL, HPL (targeting 30, 50 and 70% of plantain in sward, respectively) across production years.

Plantain Overview Massey Resowing Graph
Plantain Overview Massey Resowing Graph

Massey nitrogen leaching

Compared to a sward sown with perennial ryegrass/clover, average annual total N and nitrate leaching were significantly lower in the plantain treatments in the first two years of the Massey experiment (Table 6).

In Year 3, leaching was higher in the High PL treatment. This is likely due to the re-establishment of plantain and clover in Year 2 which resulted in very high clover (33%) and low ryegrass (13%), with 46% plantain. Higher leaching in that treatment resulted from the combination of pasture re-establishment (spraying, soil disturbance), high clover, and low ryegrass (and therefore lower winter activity) to utilise the extra nitrogen fixed from clover and mineralised from the soil. This was mitigated by oversowing with Italian ryegrass to utilise the extra nitrogen before Year 4. Leaching was numerically lower in the Low and Medium PL treatments than in the RGWC in Year 3, though treatment differences were not significant.

In Year 4, wet conditions meant low stocking densities through spring, and high amounts of silage made, to protect the paddocks from damage. The wet spring conditions were followed by a dry autumn and winter, resulting in low drainage. These conditions resulted in low leaching, masking any plantain treatment effect in Year 4. Year 5 was also characterised by low drainage, high pasture growth rates, lower than optimum stocking densities and high silage removal, also resulting in low leaching.

Table 6. Proportion of plantain in the sward and diet and corresponding nitrate and total N leached over five years at the Massey plantain grazing trial

Where superscript letters are different, there is a statistically significant treatment difference.

Year Treatment Plantain % of
DM in sward
Plantain % of
DM in diet
Total N leached
(kg N/ha)
Nitrate-N leached
(kg N/ha)
Year 1: 2019/20 Ryegrass 6a 4a 16a 11a
Low PL 29b 20b 9b 5b
Med PL 34c 28c 10b 4b
High PL 49d 35d 10b 5b
Year 2: 2020/21 Ryegrass 3a 2a 29a 25a
Low PL 32b 24b 23b 19b
Med PL 47c 35c 18c 13c
High PL 46c 34c 23b 18b
Year 3: 2021/22 Ryegrass 2a 1a 19a 10a
Low PL 20b 15b 16a 9a
Med PL 24c 18c 16a 8a
High PL 46d 34d 23b 15b
Year 4: 2022/23 Ryegrass 4a 3a 10 6
Low PL 24b 15b 9 5
Med PL 28b 17b 8 4
High PL 32c 21c 10 6
Year 5: 2023/24 Ryegrass 4a 3a 14 6
Low PL 13b 10b 13 6
Med PL 20c 13b 10 4
High PL 16bc 11b 13 6
Average: 2019/24 Ryegrass 4a 2a 18 12b
Low PL 24b 17b 14 9ab
Med PL 32c 22c 12 7a
High PL 38d 27d 16 10ab
P value* treatment <0.001 <0.001 0.1010 0.032
P value year <0.001 <0.001 <0.001 <0.001
P value treatment x year** <0.001 <0.001 0.002 <0.001

Note: Results are preliminary as we await development of peer-reviewed manuscripts.
* P value: An indication of how likely the result is due to chance. The smaller the P value, the more confident we can be that the observed difference is meaningful rather than random. Values below 0.05 are considered statistically significant.
**Treatment x year interaction: Where the treatment × year interaction is statistically significant, treatment differences were not consistent across years. In this case, results should be interpreted separately for each year rather than drawing conclusions from the overall average across years.

Massey production

There were no treatment differences in milk production. Pasture yield was similar between treatments except Year 4, which was characterised by wet winter/spring conditions and had higher pasture growth in the ryegrass/clover treatment compared to all plantain treatments.

Table 7. Estimated herbage yield (t DM/ha/yr) for each herbage treatment at Massey farmlet trial.

Ryegrass and white clover sward (RGWC), and RGWC plus Low, Medium, or High plantain.

  Herbage treatment
Season Ryegrass/clover Low plantain Medium plantain High plantain
2019/20 (Year 1*) 15.3 14.9 15.1 15.3
2020/21 (Year 2) 15.8 16.9 17.3 16.6
2021/22 (Year 3) 14.7 14.3 14.3 10.9**
2022/23 (Year 4) 14.3a 12.5b 12.9b 12.5b
2023/24 (Year 5) 16.6 15.7 15.6 15.9

Note: Different letters show significant differences (P< 0.05) between herbage treatments within year.

*Data collection started in Sept 2019 (after pastures were established in April 2019). Year 1 includes data for 9 months (Sept-May).

**Due to the high plantain treatment being re-sown in autumn of 2021, no pasture yield data for the high plantain treatment were collected in the winter of 2021. This number therefore only represents the cumulative total of spring to autumn.

Plantain Overview Massey Pasture Growth Rate Graph
Plantain Overview Massey Pasture Growth Rate Graph

Monthly pasture growth rate (kg DM/ha/day, ± standard error) for the Ryegrass White Clover (RGWC), and Low (LPL), Medium (MPL) and High (HPL) plantain treatments from Sept 2019 to May 2024.


Plantain Potency and Practice programme

The Lincoln and Massey trials are part of the Primary Sector Growth Fund Plantain Potency and Practice Programme, a seven-year collaborative research initiative led by DairyNZ and funded by the Ministry for Primary Industries, DairyNZ, PGG Wrightson Seeds and Fonterra. Research is delivered in partnership with Lincoln University, Massey University, Lincoln Agritech and the Bioeconomy Science Institute (BSI).

Visit the main programme page to learn more about the research and farmer guidance being developed to support plantain use on farm.

Lincoln and Massey publications

Lincoln

Al-Marashdeh, O., Herath, HMGP., Thomas, C., McMillan, N., Tanner, J., Woods, RR., and Bryant, RH. (2026). Calibration of a rising plate meter for herbage mass estimation in ryegrass-white clover mixed swards containing different proportions of plantain (Plantago lanceolata L.). 2026. The Journal of Agricultural Science. 1-26. doi:10.1017/S0021859626100690

Herath, G., Thomas, C., McMillan, N., Woods, R., Bryant, R., and Al- Marashdeh, O. (2023). Incorporating Plantain into Ryegrass-White Clover Mixed Sward for an Economically and Environmentally Sustainable Dairy System: Year One of a Farm System Study: Plantain-Based Pasture for Sustainable Dairy Systems. Journal of New Zealand Grasslands 85: 321–331. https://doi.org/10.33584/jnzg.2023.85.3635

Hintz, K., Woods, RR., McMillan, N., Thomas, C., Bryant, RH. and Al-Marashdeh, O. (2026). Incorporating Plantain Into a Perennial Ryegrass–White Clover Mixed Sward for an Environmentally Sustainable Dairy System: A Three-Year Farm System Study. New Zealand Journal of Agricultural Research 69(2), e70041.

Massey

Navarrete, S., Kemp, P. D., Pinxterhuis, J. B., Minnée, E. M. K., & Fransen, K. E. (2023). Evidence of environmental benefits of plantain pastures. In Proceedings of the XXV International Grassland Congress (pp. 1846-1879). https://doi.org/10.52202/071171-0453

Nguyen, T.T., Navarrete, S., Horne, D., Donaghy, D., Kemp, P. (2024). Milk production and nitrogen excretion of grazed dairy cows in response to plantain (Plantago lanceolata) content and lactation season. Animal Bioscience https://doi.org/10.5713/ab.23.0400

Nguyen, T.T., Navarrete, S., Horne, D.J., Donaghy, D.J., Bryant, R.H. and Kemp, P.D. (2023). Dairy cows grazing plantain-based pastures have increased urine patches and reduced urine N concentration that potentially decreases N leaching from a pastoral system. Animals 13, 528, 12 pages. https://doi.org/10.3390/ani13030528

Nguyen, T.T., Navarrete, S., Horne, D., Donaghy, D.J. and Kemp, P.D. (2022). Incorporating plantain with perennial ryegrass - white clover in a dairy grazing system: dry matter yield, botanical composition, and nutritive value response to sowing rate, plantain content and season. Agronomy 12, 2789, 14 pages https://doi.org/10.3390/agronomy12112789

Nguyen, T.T., Navarrete, S., Horne, D.J., Donaghy, D.J., and Kemp, P.D. 2022. Effect of plantain content in ryegrass-based dairy pastures on nitrate leaching and key components of the nitrogen cycle. In: Adaptive Strategies for Future Farming. (Eds. C.L. Christensen, D.J. Horne and R. Singh). http://flrc.massey.ac.nz/publications.html Occasional Report No. 34. Farmed Landscapes Research Centre, Massey University, Palmerston North, New Zealand.

Sivanandarajah, K., Molano, G., Donaghy, D., Horne, D., Kemp, P., Navarrete, S., Ramilan, T., & Pacheco, D. (2024). Plantain-mixed pasture collected in different climatic seasons produced less methane and net ammonia than ryegrass-white clover pasture. 20 Years of ADSS: timeless practices, re-innovation, and future thinking. Australasian Dairy Science Symposium 2024, Christchurch, New Zealand. https://doi.org/10.1071/AN24287

Sivanandarajah, K., Donaghy, D., Kemp, P., Navarrete, S., Horne, D., Ramilan, T., Molano, G., & Pacheco, D. (2025). Effects of Plantain (Plantago lanceolata L.) Metabolites Aucubin, Acteoside, and Catalpol on Methane Emissions In Vitro. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/acs.jafc.4c12140

Wilson S, Donaghy D, Horne D, Navarrete S, Kemp P. (2024). Investigating the impact of treading damage on the plantain (Plantago lanceolata L.) content and performance of a plantain/perennial ryegrass (Lolium perenne L.) pasture over two years. Journal of New Zealand Grasslands, 31, 97-107. https://dx.doi.org/10.33584/jnzg.2024.86.3682

Wilson, S.S., Donaghy, D. J., Horne, D. J., Navarrete, S., & Kemp, P. D. (2024). An inexpensive and effective method for simulating cattle treading damage on pasture. Opportunities for improved farm and catchment outcomes. Occasional Report No. 36. Farmed Landscapes Research Centre, Massey University, Palmerston North, New Zealand. https://www.massey.ac.nz/~flrc/workshops/24/Manuscripts/Wilson_Sam.pdf

Wilson, S., Donaghy, D., Horne, D., Navarrete, S., & Kemp, P. (2023). Plantain (Plantago lanceolata L.) content within plantain/ perennial ryegrass (Lolium perenne L.) pastures may be limited by livestock treading damage. Journal of New Zealand Grasslands, 85, 177–186. https://doi.org/10.33584/jnzg.2023.85.3634

Wilson, S.; Donaghy, D.; Horne, D.; Navarrete, S.; Kemp, P.; Rawlingson, C. Plantain (Plantago lanceolata L.) Leaf Elongation and Photosynthesis Rates Are Reduced under Waterlogging. Biol. Life Sci. Forum 2023, 27, 26. https://doi.org/10.3390/IECAG2023-14976

Wilson, S. S., Donaghy, D. J., Horne, D. J., Navarrete, S., & Kemp, P. D. Plantain (Plantago lanceolata L.) Growth is limited under waterlogging., in Proceedings of the International Grasslands Congress, May 2023: Kentucky, USA. UKnowledge - IGC Proceedings (1997-2023): Plantain (Plantago lanceolata L.) Growth Is limited Under Waterlogging (uky.edu)

Vi, C.; Kemp, P.D.; Saggar, S.; Navarrete, S.; Horne, D.J. (2023). Effective Proportion of Plantain (Plantago lanceolata L.) in Mixed Pastures for Botanical Stability and Mitigating Nitrous Oxide Emissions from Cow Urine Patches. Agronomy, 13, 1447. https://doi.org/10.3390/agronomy13061447

Vi. C, Kemp P., Saggar S., Navarrete S., Horne D., 2022. Plantain mixed pastures: Botanical composition and nitrous oxide emissions for cow urine patches. In: Adaptive Strategies for Future Farming. (Eds. C.L. Christensen, D.J. Horne and R. Singh). http://flrc.massey.ac.nz/publications.html. Occasional Report No. 34. Farmed Landscapes Research Centre, Massey University, Palmerston North, New Zealand. 12 pages

Additional resources

Plantain Potency and Practice programme

/research/science-projects/plantain-potency-and-practice-programme/

Plantain case studies

/research/case-studies/?page=1&tags=Plantain

Plantain overview

/feed/crops/plantain-overview/

Assessing Plantain

/feed/crops/assessing-plantain/
Last updated: Jul 2026
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